Background and purpose: New coronavirus disease 2019 (COVID-19) can cause persistent symptoms and physical weakness that can lead to a limitation in activities of daily living (ADL). There is a lack of evidence about the performance in the six-minute step test (6MST) of post-COVID-19 patients and healthy subjects. The aim of this study is to investigate the cardiorespiratory response induced by the 6MST in post-COVID-19 patients and compare it with the response of six-minute walk test (6MWT). Methods: this cross-sectional study was conducted on 34 post-COVID-19 patients and 33 healthy subjects. Assessment was performed at one month from a non-severe SARS-CoV-2 infection. Both groups were assessed by using 6MST, 6MWT, and pulmonary function test (PFT). PCFS scale was used for post-COVID-19 group to assess functional status. Physiological responses: heart rate (HR), respiratory rate (RR), oxygen saturation (SpO2), blood pressure (BP), and Borg scale for fatigue and dyspnea were recorded before and after 6MST and 6MWT. Results: the performance of the post-COVID-19 group was worse than the healthy group in both tests. In 6MWT, the distance walked by the post-COVID-19 group (423±7) was 94m lesser than the healthy group, and the number of climbed steps in 6MST (121±4) was 34 steps lesser than the healthy group. Both results were statistically significant (p < 0.001). There was a moderate positive correlation between 6MST and 6MWT in walked distance versus steps number in post-COVID-19 group (r=0.5, p=0.001). In addition, there was moderate to strong correlation between the two tests in post (SBP, DBP, dyspnea, and fatigue) with p ≤ 0.001. Conclusions: Six-minute step test produced similar cardiorespiratory responses when compared to a 6MWT. The 6MST can be used as an assessment tool for COVID-19 patients to evaluate their functional capacity and ADL.