Background: Evidence-based practice (EBP) is an important factor determining the quality of healthcare. The field of physiotherapy is still limited by indirect access in several countries including the United Arab Emirates (UAE) which creates added pressure to justify the merit in its practitioner's capabilities. EBP remains a critical yet less understood subject among physiotherapists worldwide. This study aims to explore the behaviour, attitudes, awareness and knowledge towards EBP among practicing physiotherapists in the UAE. It also enquires about their perception of the barriers in the implementation of EBP. Design and Methods: A descriptive cross-sectional study design was employed. The questionnaire survey developed by Alshehri et al. (2017) was used in this study. The survey consists of 15 close-ended questions and divided into 7 sections. Two hundred and fiftyeight physiotherapists responded to the web-based survey. Data were analyzed using IBM SPSS version 22. Results: Results showed that the awareness of EBP is limited to a few terms including EBP, systematic literature review, and random trials while other terms associated with scientific studies are not known well. The attitude towards EBP was found to be significantly related to the knowledge of EBP (r = 0.208) and the perception of barriers to it (r = 0.156). The behaviour towards EBP was found positively related to its knowledge (r=0.134) and the perception of barriers (r= 0.216). The physiotherapists prefer to use their own experience, books and research articles to apply EBP but do not consider collaborative practice with peers as valuable source of EBP. However, their attitudes towards EBP are largely positive though their perception of barriers grows with better knowledge and understanding of EBP. The barriers in the implementation of EBP are a lack of research knowledge and skills, time, support, and resources which indicate opportunities for the decision-makers to improve the adoption of EBP among these professionals. Conclusion: This