Background: Non-specific musculoskeletal pain is becoming more prevalent all over the world. Physical inactivity is a significant contributor to the increased prevalence of non-specific musculoskeletal pain conditions among the young working population. Musculoskeletal pain in multiple body regions has also been linked to a lower health-related quality of life. There is limited research on the prevalence of non-specific musculoskeletal pain in young working people and its relationship to physical activity levels and health- related quality of life in the UAE population. Objective: We aimed to administer a cross sectional survey with two objectives: (1) to determine the prevalence of non-specific work related musculoskeletal (WRMSK) pain among working UAE adults (2) to study the association of nonspecific work musculoskeletal pain conditions and the physical activity levels and health-related quality of life category scores across gender and age groups. Methods: Five hundred and seven participants of the age groups 18-28 and 29-39 years were administered the Nordic Musculoskeletal Questionnaire (NMQ), the Global Physical Activity Questionnaire (GPAQ), and the World Health Organization Quality of Life Questionnaire. The prevalence rate of non-specific musculoskeletal pain was calculated across the gender and age groups. Chi- square test was used to determine the association of occurrence of non-specific musculoskeletal pain with physical activity levels and WHO BREF quality of life scores. Results: The prevalence of non-specific musculoskeletal pain was found to be very high in the lower back (48%), followed by the shoulders (41%) and neck (40%), with an overall prevalence rate of 74.8%. The physical activity levels were moderate to high among the participants and health-related quality of life scores indicated good quality of life among young adults population in the UAE society. The results from the chi-square test indicated that the physical activity levels and health-related quality of life